Comparative biochemical studies of rapamycin and wortmannin on transforming growth factor-beta 1 (TGF-beta1)-mediated G(1)-S cell cycle progression in C3H-10T1/2 embryonic fibroblasts.

Chapter 1. Introduction

• The Mammalian Cell Cycle

• TGF-β-Mediated Signaling and Modulation of Peptide Growth Factors

• Extracellular Signals in G1-S Phase Progression

• Accelerating and Braking Components of the Mammalian Cell Cycle Machinery

• Roles of Cell Cycle Components in Development, Tumorigenesis, and Waning of Proliferative Potential

• The Cancer Challenge

• Literature Cited

Chapter 2. Materials and Methods

• Cell Culture, Calculation of Generation Time

• Preparation of Radiolabeling Solution, Radiolabeling Assay, System A Amino Acid Transport Measurement

• Isolation of Whole Cell Extract, Immunoblot Analysis

Chapter 3. Density Differential Responses of Normal Versus Transformed 10T½ Murine Embryonic Fibroblasts

• Summary and Introduction

• Materials and Methods

• Results and Discussion

• Literature Cited

Chapter 4. P27kip1 Down-Regulation Was Not an End-Point in Rapamycin Inhibition of Transforming Growth Factor (TGF)-β1-Stimulated Cell Cycle Progression in C3H-10T½ Murine Embryonic Fibroblasts

• Summary and Introduction

• Materials and Methods

• Results

• Discussion

• Literature Cited

Chapter 5. Transforming Growth Factor (TGF)-β1-Mediated Regulation of Cell Cycle-Sensitive Translational Components: Regulation of P70 S6 Kinase and Eukaryotic Translational Initiation Factor (EIF)-2α

• Abstract and Introduction

• Materials and Methods

• Results

• Discussion

• Literature Cited

Chapter 6. Cell Cycle Kinetic Profiles for Rapamycin and Wortmannin Effect on TGF-β1-Mediated DNA Synthesis, Protein Synthesis, and System A Neutral Amino Acid Transport in C3H-10T½ Murine Embryonic Fibroblasts

• Introduction

• Materials and Methods

• Results

• Discussion

• Literature Cited

Conclusions